OF-1

Alternative_name

4-Bromo-N-(2,3-dihydro-6-methoxy-1,3-dimethyl-2-oxo-1H-benzimidazol-5-yl)-2-methylbenzenesulfonamide
Description

OF-1 is a selective BRPF1B and BRPF2 bromodomain inhibitor (Kd values are 100 and 500 nM respectively). OF-1 displays 39-fold selectivity for BRPF1B and BRPF2 over BRD4. It increases thermal stability of full length BRPF1B at 1 µM, in the cellular thermal shift assay. It also demonstrates accelerated fluorescence recovery after photobleaching (FRAP) at 5 µM in the BRPF2 FRAP assay. OF-1 displays modest general cytotoxicity.
CAS Number

919973-83-4
Structure Available

Yes
Salt Form

No
Molecular Formula

C₁₇H₁₈BrN₃O₄S
Molecular Weight

440.31
Cell Permeable

Yes
Purity

≥98% by HPLC
Solubilities

DMSO (>8 mg/ml)
Handling

Protect from air and light
Tag Line

A selective BRPF1B and BRPF2 bromodomain inhibitor
Storage Condition

-20°C
Shipping Condition

gel pack
Shelf Life

36 months
PubChem CID

35397514
PubChem CID Link
SMILES

CC1=C(C=CC(=C1)Br)S(=O)(=O)NC2=C(C=C3C(=C2)N(C(=O)N3C)C)OC
InChi

InChI=1S/C17H18BrN3O4S/c1-10-7-11(18)5-6-16(10)26(23,24)19-12-8-13-14(9-15(12)25-4)21(3)17(22)20(13)2/h5-9,19H,1-4H3
InChi Key

YUNQZQREIHWDQT-UHFFFAOYSA-N
Data Sheet Link
MSDS Available

Yes

Gene target

OF-1
Gene symbol

SPI1, BRIP1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
Short name

OF-1
Alternative name

on-1

Identity

HGNC:11241
Gene

SPI1
Long gene name

Spi-1 proto-oncogene
Synonyms gene name
- spleen focus forming virus (SFFV) proviral integration oncogene
Synonyms
- PU.1
- SPI-A
- OF
- SFPI1
- SPI-1
Synonyms name
- hematopoietic transcription factor PU.1
- 31 kDa transforming protein
GenBank acession
- X52056
Locus

11p11.2
Discovery year

1988-07-04
Entrez gene record

6688
Pubmed identfication
- 1693183
RefSeq identity
- NM_003120
Classification
- ETS transcription factor family
VEGA ID

OTTHUMG00000150150

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081