CD300C, human recombinant
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Catalog number7312-100
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PricePlease ask
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Size100 ug
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SynonymsCMRF35-like molecule 6, CLM-6, CMRF-35, CMRF-35A, CMRF35, CMRF35-A1, CMRF35A, CMRF35A1, IGSF16, LIR
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Alternative_namesCMRF35-like molecule 6, CLM-6, CMRF-35, CMRF-35A, CMRF35, CMRF35-A1, CMRF35A, CMRF35A1, IGSF16, LIR
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DescriptionPresent on monocytes, neutrophils, and some T and B lymphocytes
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥85%
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AssaySDS-PAGE
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Endotoxin Level< 1.0 EU per 1 microgram of protein
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Molecular Weight20.2 kDa (186 aa, 21-183 aa + His tag), confirmed by MALDI-TOF.
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Storage Temp-80°C
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ShippingDry Ice
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form description1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M Urea and 10% glycerol.
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Background InformationCMRF35-like molecule 6, also known as CD300C, belongs to the CD300 family. The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes. Recombinant human CD300C protein, fused to His-tag at N-terminus, was expressed in E.coli.
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Amino acid sequenceMGSSHHHHHH SSGLVPRGSH MGSGYFPLSH PMTVAGPVGG SLSVQCRYEK EHRTLNKFWC RPPQILRCDK IVETKGSAGK RNGRVSIRDS PANLSFTVTL ENLTEEDAGT YWCGVDTPWL RDFHDPIVEV EVSVFPAGTT TASSPQSSMG TSGPPTKLPV HTWPSVTRKD SPEPSPHPGS LFSNVR
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCD300C
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Short nameCD300C, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameCD300c molecule, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetCD300c molecule, CLM-6 and CMRF-35 and CMRF-35A and CMRF35 and CMRF35-A1 and CMRF35A and CMRF35A1 and IGSF16 and LIR, CD300C and IDBG-67336 and ENSG00000167850 and 10871, protein binding, Plasma membranes
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Gene info
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Identity
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Gene
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Long gene nameCD300c molecule
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Synonyms gene name
- CD300c antigen
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-02-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data