-
Long name
KiSS1 receptor Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
-
Also known as
Anti-KiSS1 receptor PAb ALEXA FLUOR 488
-
Category
Conjugated Primary Antibodies
-
Conjugated with
ALEXA FLUOR® 488
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
KiSS1 receptor
-
Specificity
This is a highly specific antibody against KiSS1 receptor.
-
Modification
Unmodified
-
Modification Site
None
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Immunogen range
139-157/398
-
Subcellular location
Cytoplasm
-
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human KiSS1 receptor
-
Gene ID Number
84634
-
Swiss Prot
Q969F8
-
Tested applications
IF(IHC-P)
-
Recommended dilutions
IF(IHC-P)(1:50-200)
-
Crossreactivity
Human, Mouse, Rat
-
Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Background of the antigen
Receptor for metastin (kisspeptin-54 or kp-54), a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.
-
Purification
Purified by Protein A.
-
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
-
Excitation emission
499nm/519nm
-
Synonyms
HH8; CPPB1; GPR54; AXOR12; KISS-1R; HOT7T175; KiSS-1 receptor; G-protein coupled receptor 54; G-protein coupled receptor OT7T175; Hypogonadotropin-1; Kisspeptins receptor; Metastin receptor; KISS1R
-
Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-KiSS1 receptor has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Conjugation
Alexa Fluor
-
Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
-
French translation
anticorps