Human ICA1(Islet Cell Autoantigen 1) ELISA Kit

• Catalog number
  ELK5196
• Price
  Please ask
• Size
  96T

• Alternative Names
  ICA69; ICAp69; ICA-p69; 69 kDa islet cell autoantigen
• Assay Type
  Sandwich
• Sensitivity
  0.068ng/mL
• Standard
  10ng/mL
• Detection range
  0.156-10ng/mL
• Assay length
  3h
• Research Area
  Immune molecule;
• Test principle
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Islet Cell Autoantigen 1 (ICA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Islet Cell Autoantigen 1 (ICA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Islet Cell Autoantigen 1 (ICA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Islet Cell Autoantigen 1 (ICA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Description
  For cells, cell lines and tissues in culture till half confluency.
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

• Gene target
  ICA1   Islet   Autoantigen  
• Gene symbol
  ICA1-AS1, ICA1
• Short name
  ICA1(Islet Autoantigen 1) ELISA Kit
• Technique
  ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
• Host
  antigen assay ELISA kit 1 Kit (96 Wells)
• Species
  Human, Humans
• Alternative name
  H. sapiens islet cell autoantigen 1, 69kDa(Islet cellular Autoantigen 1) Enzyme-linked immunosorbent assay reagent
• Alternative technique
  elisas, kits
• Alternative to gene target
  islet cell autoantigen 1, 69kDa, ICA69 and ICAp69, ICA1 and IDBG-8551 and ENSG00000003147 and 3382, protein domain specific binding, Cell surfaces, Ica1 and IDBG-128452 and ENSMUSG00000062995 and 15893, BT.35115 and IDBG-629525 and ENSBTAG00000000799 and 535346
• Tissue
  cell

Gene info

• Identity
  HGNC:55606
• Gene
  ICA1-AS1
• Long gene name
  ICA1 antisense RNA 1
• Locus
  7p21.3
• Discovery year
  2021-06-09
• Entrez gene record
  100505938
• RefSeq identity
  • NR_125740
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:5343
• Gene
  ICA1
• Long gene name
  islet cell autoantigen 1
• Synonyms gene name
  • islet cell autoantigen 1 (69kD)
  • islet cell autoantigen 1, 69kDa
• Synonyms
  • ICAp69
  • ICA69
• Locus
  7p21.3
• Discovery year
  1992-08-24
• Entrez gene record
  3382
• Pubmed identfication
  • 7918678
  • 8777998
• RefSeq identity
  • NM_004968
• Classification
  • AH domain containing
• VEGA ID
  OTTHUMG00000152008

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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