Mouse Anti-Mouse HCN3 Monoclonal IgG1, Clone S141-28
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Catalog numberSMC-306D-A565
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PricePlease ask
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Size100 µg
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CloneS141-28
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ImmunogenFusion protein amino acids 660-779 (C terminus) of mouse HCN3
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Antibody s full descriptionMouse Anti-Mouse HCN3 Monoclonal IgG1 Antibody, Clone: S141-28: ATTO 565
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameKIAA1535 Antibody, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 Antibody, hyperpolarization activated cyclic nucleotide-gated potassium channel 3 Antibody
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Raised inMouse
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Antibody s targetHCN3
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Primary research fieldsNeuroscience, Ion Channels, Cyclic Nucleotide-Gated Ion Channels, cardiovascular System, Heart
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF , AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~90kDa. No cross-reactivity against other HCNs.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-306 was sufficient for detection of HCN3 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextHyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (1).
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Bibliography1. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34233.
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Released date6-Jul-2009
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_032253.1
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Gene number15168
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Protein numberO88705
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-HCN3 Monoclonal Antibody, Clone S141-28 (SMC-306). Tissue: brain tissue. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN3 Monoclonal Antibody (SMC-306) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Granular staining of some Purkinje cells. Staining appears to be focal. Western Blot analysis of Rat brain membrane lysate showing detection of HCN3 protein using Mouse Anti-HCN3 Monoclonal Antibody, Clone S141-28 (SMC-306). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN3 Monoclonal Antibody (SMC-306) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-HCN3 Monoclonal Antibody, Clone S141-28 (SMC-306). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN3 Monoclonal Antibody (SMC-306) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Mouse Anti-HCN3 Antibody [S141-28] used in Immunohistochemistry (IHC) on Mouse brain tissue (SMC-306) Mouse Anti-HCN3 Antibody [S141-28] used in Western Blot (WB) on Rat brain membrane lysate (SMC-306) Mouse Anti-HCN3 Antibody [S141-28] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-306)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolHCN3, IGHV4-28, SNORD114-28, RNVU1-28, SNORD116-28, SNORD115-28, ERVW-28, ERVK-28, IGKV2-28, IGHVII-28-1
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Short nameMouse Anti-Mouse HCN3 Monoclonal IgG1, Clone S141-28
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 565
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse HCN3 monoclonal IgG1, clonality S141-28
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Alternative techniquemurine, antibodies
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Clone nameS141-28
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Gene info
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Identity
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Gene
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Long gene namehyperpolarization activated cyclic nucleotide gated potassium channel 3
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Synonyms gene name
- hyperpolarization activated cyclic nucleotide-gated potassium channel 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-09-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cyclic nucleotide gated channels
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 4-28
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-28
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 28
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Locus
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Discovery year2019-07-26
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-28
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-28
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Synonyms
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Locus
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Discovery year2011-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 28
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Synonyms gene name
- endogenous retrovirus group W, member 28
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 28
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Synonyms gene name
- endogenous retrovirus group K, member 28
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GenBank acession
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Locus
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Discovery year2014-01-03
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-28
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-28-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-28-1
- immunoglobulin heavy variable (II)-28-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
