Human Caspase 8 PicoKine ELISA Kit

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• Catalog number
  EK1426
• Price:

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• Size
  1x96 well plate

# #

• ELISA s application
  For quantitative measurement of human Caspase 8 in cell culture supernatant, tissue homogenates and serum.
• Gene tested
  human Caspase 8
• ELISA standard curve
  Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.003 + 0.067 + 0.111 + 0.218 + 0.407 + 0.820 + 1.339 + 2.191
• ELISA kit detection limits
  31.2pg/ml to 2000pg/ml
• ELISA s sensitivity
  < 10pg/ml
• Target of this ELISA
  Natural and recombinant human Caspase 8
• Cross reaction
  the ELISA does not cross react with any other proteins except from closely related species
• ELISA s precision parameters
  intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);153;589;1527;170;836;1721 ELISA standard deviations 6.43;28.86;80.93;11.05;59.36;141.12 CV(%);4.2;4.9;5.3;6.5;7.1;8.2
• ELISA kit description
  GENTAUR's human Caspase 8 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Caspase 8 was used as capure antibodies on the ELISA test plate. The internal standards (Expression system for standard: E. coli, Immunogen sequence: S217-D384 & L385-D479) and your samples are measured with a biotin conjugated polyclonal from goat specific for Caspase 8 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human Caspase 8 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
• Detector and capure antibodies
  a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
• The ELISA contains
  Catalog number;Description;Quantity -;96-well plate precoated with anti- human Caspase 8 antibodies;1 ST0000-10;Lyophilized recombinant human Caspase 8 standard;10ng/tube×2 AR1107;Biotinylated anti- human Caspase 8 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
• Anti coagulation
  /
• How the elisa works
  Sample the amount of 100ul/well of the standard solution at 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human Caspase 8 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, tissue homogenates or serum to the ELISA plate. Keep in mind that each human Caspase 8 standard solution and each sample be measured in duplicate.
• Minutes of TMB incubation
  20-25
• More gene info
  Caspase-8 is a caspase protein, encoded by the CASP8 gene. It is mapped to human chromosome 2q33-q34 and mouse chromosome 1B-proximal C. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. And this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
• Antigen
  E.coli,S217-D384&L385-D479
• Keep at
  Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
• Uniprot
  Caspase-8
• Gene name
  caspase 8
• Alternative
  CASP-8;CASP8;CASP 8;Caspase-8;Caspase8;Caspase 8;MACH;MCH5;MCH 5;CAP 4;CAP4;CAP-4;FADD Like ICE
• Gen protein identity
  Q14790
• Entrez nr
  841
• Description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

#

• Gene target
  Caspase   PicoKine  
• Gene symbol
  CASP8
• Short name
  Caspase 8 PicoKine ELISA Kit
• Technique
  ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
• Host
  antigen assay ELISA kit 1 Kit (96 Wells)
• Species
  Human, Humans
• Alternative name
  H. sapiens caspase 8 PicoKine Enzyme-linked immunosorbent assay reagent
• Alternative technique
  elisas, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:1509
• Gene
  CASP8
• Long gene name
  caspase 8
• Synonyms gene name
  • caspase 8, apoptosis-related cysteine protease
  • caspase 8, apoptosis-related cysteine peptidase
• Synonyms
  • MCH5
  • MACH
  • FLICE
  • Casp-8
• GenBank acession
  • U60520
• Locus
  2q33.1
• Discovery year
  1996-11-11
• Entrez gene record
  841
• Pubmed identfication
  • 8681376
  • 8681377
• RefSeq identity
  • NM_001228
• Classification
  • Caspases
  • Death effector domain containing
  • Death inducing signaling complex
  • Ripoptosome
• VEGA ID
  OTTHUMG00000132821
• Locus Specific Databases
  • CASP8base: Mutation registry for Caspase 8 deficiency
  • LRG_34

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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