pCR-BluntII-TOPO-HAND2 Plasmid
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Catalog number
PVTB00244S
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Price
381.42 USD
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Size
2 ug
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbol
HAND2-AS1, HAND2
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Short name
pCR-BluntII-TOPO-HAND2 Plasmid
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Technique
plasmid, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. plasmids in 1
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Alternative name
PCR test kit-BluntII-TOPO-heart and neural crest derivatives expressed 2 Plasmid
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Alternative technique
plasmids, dna-amplification
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Alternative to gene target
heart and neural crest derivatives expressed 2, bHLHa26 and dHand and DHAND2 and Hed and Thing2, HAND2 and IDBG-44790 and ENSG00000164107 and 9464, E-box binding, nuclei, Hand2 and IDBG-156149 and ENSMUSG00000038193 and 15111, HAND2 and IDBG-629312 and ENSBTAG00000044123 and 781874
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Gene info
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Identity
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Gene
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Long gene name
HAND2 antisense RNA 1
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Synonyms gene name
- HAND2 antisense RNA 1 (head to head)
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Synonyms
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Synonyms name
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Locus
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Discovery year
2013-07-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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