Mammalian Cell Extraction Kit

Summary

• Species reactivity- Mammalian • Applications- Enzyme activity assays (e.g., caspase activity assays), Western blot analysis, and others.
Sample Type

Cell cultures (adherent and suspension), fresh and frozen tissues
Features Benefits

• Simple procedure; takes less than 20 minutes • Fast and convenient • The Mammalian Cell Extraction Kit provides optimized lysis buffer and protease inhibitor cocktail as well as other reagents for extraction of mammalian proteins from tissues and cells under nondenaturing conditions.
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

The Mammalian Cell Extraction Kit provides optimized cell extraction buffer, protease inhibitor cocktail, and DTT for convenient extraction of mammalian proteins from cultured cells and tissue samples, under nondenaturing conditions. Cell lysate prepared using the kit can be used in a variety of applications, such as enzyme activity assays (e.g., caspase activity assays), Western blot analysis, and others. The entire procedure takes less than 20 minutes.
Data Sheet Link
Additional description

For cells, cell lines and tissues in culture till half confluency.

Gene target

Mammalian Extraction Kit
Short name

Mammalian Extraction Kit
Alternative name

Mammalian cellular Extraction reagent
Alternative technique

kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Tissue

cell