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HSP70 ELISA Kit

HSP70 ELISA Kit is available 6 times from Stressmark kits labs

SKT-105-480 | HSP70 ELISA Kit size: 5 x 96 well | 2,656.26 USD

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SKT-105-96 | HSP70 ELISA Kit size: 96 well | 667.38 USD

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SKT-108-480 | HSP70 ELISA Kit (High-Sensitivity) size: 5 x 96 well | 3,180.24 USD

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SKT-108-96 | HSP70 ELISA Kit (High-Sensitivity) size: 96 well | 790.92 USD

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SKT-124-96 | HSP70 ELISA Kit (PLANT) size: 96 well | 827.33 USD

Catalog number SKT-124-96
Price 827.33 USD
Size 96 well
Kit's description Colorimetric detection used to quantitate HSP70 in plants
Scientific context HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at more.
Brief protocol 1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard to appropriate wells. 3. Cover plant with Plate Sealer and incubate at room temperature for 1 hour. 4. Wash plate four times with Wash Buffer. 5. Add 100 µL of Detection Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer as described in step 4. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer as described in step 4. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Kit contents Anti-Hsp70 Immunoassay Plate, 5X Hsp70 Extraction Reagent, Recombinant Hsp70 (Alfalfa) Standard, Standard and Sample Diluent, 10X Wash Buffer Concentrate, Anti-Hsp70 Biotinylated Antibody Concentrate, Anti-Hsp70 Biotinylated Antibody Diluent, Streptavidin: HRP Concentrate, Streptavidin: HRP Diluent, TMB Substrate, Stop Solution
Assay cross-reactivity Plant, Human
Samples to be used with this kit Plant extract, Cell Lysates, Tissue, Serum, Plasma
Kit's sensitivity 0,18 ng/ml
Assay detection limit 1,563 - 100 ng/ml
Bibliography 1. Boorstein W.R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38(1): 1-17. 2. Rothman J. (1989) Cell. 59: 591-601. 3. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 4. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 5. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
Release date 1-Oct-2012
PubMed number Refer to PubMed
Tested applications No data available / currently testing
Tested reactivity No data available / currently testing
Product image legend Typical Standard Curve for the HSP70 ELISA Kit (PLANT) (Enzyme-Linked Immunosorbent Assay) StressXpress® - SKT-124. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 1.563 - 100 ng/mL. Graph of the Typical Standard Curve for the HSP70 ELISA Kit (PLANT) StressXpress - SKT-124
Net weight (g) 500
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About Plants RNA, DNA , cDNA and plant tissues are used to study plant genes.
Gene targetHSP70
Short name HSP70 ELISA Kit ( )
Species Plant
Alternative name HSP70 Enzyme-linked immunosorbent assay reagent (vegetal)
1. Gene info
Identity5233
GeneHSPA1B
Synonyms gene name
  • heat shock 70kD protein 1B
  • heat shock 70kDa protein 1B
Synonyms
Locus6p21.33
Discovery year1995-08-11
Pubmed identfication
Havana BLAST/BLATOTTHUMG00000031202
2. Gene info
Identity29526
GeneHSPA14
Synonyms gene name
  • heat shock 70kDa protein 14
GenBank acession
Locus10p13
Discovery year2004-04-20
Pubmed identfication
RefSeq identity
MeSH Data
Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
Product images
    #Plant#.PNG

SKT-124-480 | HSP70 ELISA Kit (PLANT) size: 5 x 96 well | 3,337.98 USD

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Stock availability In Stock
Protein target HSP70
Primary research area Cancer, Heat Shock
Category Assay ELISA Kits
Other name HSP70 1 ELISA Kit, HSP70 2 ELISA Kit, HSP70.1 ELISA Kit, HSP72 ELISA Kit, HSPA1 ELISA Kit, HSPA1A ELISA Kit, HSPA1B ELISA Kit
Brand name StressXpress®
Detection system Colorimetric Assay
Assay format Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Main intended usage ELISA kit used to quantitate HSP70 concentration in samples.
Assay precision Contact us
Maximum samples to be used with this kit 40 samples in duplicate
Assay duration 30 minutes
Storage recommendations 4°C
Shipping recommendations Blue Ice
Resources available upon request Kit Booklet, MSDS
Warnings Non-hazardous
Country of production Canada
Total weight (kg) 2
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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