Bacterial DNA Purification Kit II
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Catalog number
K1457-250
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Price
Please ask
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Size
250 Preps
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Description
Using glass beads and spin columns
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Summary
High-quality DNA is purified from bacteria using spin columns
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Detection Method
Bacteria is lysed using lysozyme, glass beads and DNA is purified using spin columns.
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Applications
PCR, restriction digestion, and hybridization
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Sample Type
Bacteria
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Features Benefits
Many downstream applications such as PCR, restriction digestion, and hybridization Rapid, easy and convenient Highly pure, high yield High quality spin columns
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Storage Conditions
Multiple Temperatures
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
BioVision's Bacterial DNA Purification Kit II provides a rapid and reliable method for isolating high-quality total DNA from a wide variety of bacterial species using spin columns. This kit will isolate all cellular DNA, including plasmid DNA. Up to 1x109 bacterial cells can be processed using a spin column. In this kit, bacterial cells in log-phase are harvested and the bacterial cell wall is digested using lysozyme and Proteinase K. The lysed samples are applied to the spin column. The DNA efficiently binds to the spin column, while proteins and other contaminants are removed. Pure DNA is then eluted in sterile water or elution buffer. Each spin column can bind approximately 100 μg genomic DNA. DNA purified using this kit can be used for downstream applications such as PCR, restriction digestion, and hybridization techniques.
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Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Bacterial DNA Purification Kit II
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Technique
Purification, dna, purified
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Alternative name
Bacterial Desoxyribonucleic acid purification reagent II
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Alternative technique
purifications, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Radioimmunoassay of proteins using antibody coupled to an immunosorbent.
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Tree numbers
- E05.478.566.380.830
- E05.478.566.639.830
- E05.601.470.380.830
- E05.601.470.639.830
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
Product images
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