QTRT1 Polyclonal Antibody

• Catalog number
  CAC07582
• Price
  Please ask
• Size
  100 μg

• Other name
  Queuine tRNA-ribosyltransferase catalytic subunit 1,Guanine insertion enzyme,tRNA-guanine transglycosylase,QTRT1,TGT,TGUT
• Immunogen
  Recombinant human Queuine tRNA-ribosyltransferase catalytic subunit 1 protein (211-403AA)
• Verified reactivity
  Human
• Verified applications
  ELISA,WB,IHC. Recommended dilution:WB:1:500-2000,IHC:1:20-1:200
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Protein number
  Q9BXR0
• Gene number
  Please refer to GenBank
• NCBI number
  Please refer to NCBI
• Purity
  Caprylic Acid Ammonium Sulfate Precipitation Purified
• Storage recommendation
  Aliquot and store at -20°C. Minimize freezing and thawing.
• Use before
  1 year
• Shipping requirements
  Blue ice
• Estimated production time
  3-7 business days
• Supplementary information
  Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).
• Notes
  For research use only. Not for diagnostic procedures.
• Hyperlink
• Properties
  If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  QTRT1  
• Gene symbol
  QTRT1
• Short name
  QTRT1 Polyclonal Antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Label
  Unconjugated
• Alternative name
  QTRT1 polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:23797
• Gene
  QTRT1
• Long gene name
  queuine tRNA-ribosyltransferase catalytic subunit 1
• Synonyms gene name
  • queuine tRNA-ribosyltransferase 1
• Synonyms
  • TGT
• Synonyms name
  • tRNA-guanine transglycosylase
• GenBank acession
  • AF302783
• Locus
  19p13.3
• Discovery year
  2003-12-10
• Entrez gene record
  81890
• Pubmed identfication
  • 20354154
• RefSeq identity
  • NM_031209
• VEGA ID
  OTTHUMG00000180588

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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