Hsp27 Antibody

• Catalog number
  3093-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Hsp27
• Target Alternative Name
  HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25
• Antibody Type
  Polyclonal
• Species Reactivities
  Rat
• Immunogen Sequence
  Synthetic peptide surrounding amino acid 193 of rat Hsp2
• Accession
  P25446
• Gene Id
  24471
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 27 kDa rat Hsp27. Rat kidney tissue lysate can be used as a positive control.
• Description
  Hsp27 exhibits a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Hsp27  
• Gene symbol
  SAFB, HSPB1
• Short name
  Hsp27 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Hsp27 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10520
• Gene
  SAFB
• Long gene name
  scaffold attachment factor B
• Synonyms
  • HET
  • SAFB1
• Synonyms name
  • Hsp27 ERE-TATA binding protein
• GenBank acession
  • L43631
• Locus
  19p13.3
• Discovery year
  1998-01-27
• Entrez gene record
  6294
• Pubmed identfication
  • 9605873
  • 8600450
• Classification
  • RNA binding motif containing
• VEGA ID
  OTTHUMG00000180502

Gene info

• Identity
  HGNC:5246
• Gene
  HSPB1
• Long gene name
  heat shock protein family B (small) member 1
• Synonyms gene name
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
• Synonyms
  • HSP27
  • HSP28
  • Hs.76067
  • Hsp25
  • CMT2F
• GenBank acession
  • X54079
• Locus
  7q11.23
• Discovery year
  1991-07-09
• Entrez gene record
  3315
• Pubmed identfication
  • 2243808
  • 9344682
• Classification
  • Small heat shock proteins
• VEGA ID
  OTTHUMG00000023228
• Locus Specific Databases
  • Inherited Peripheral Neuropathies Mutation Database
  • LRG_248

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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