HSP27 Antibody
-
Catalog numberR31650
-
PricePlease ask
-
Size0.1mg
-
-
CategoryAntibody
-
Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
-
FormAntigen affinity purified
-
ConjugationUnconjugated
-
ClonePolyclonal antibody
-
Recognised antigenHSP27
-
Host animalRabbit (Oryctolagus cuniculus)
-
ClonalityPolyclonal (rabbit origin)
-
Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applicationsWB, IHC-P, IHC-F
-
Recommended dilutionsWestern blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml
-
NotesThe stated application concentrations are suggested starting amounts. Titration of the HSP27 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
-
Intented useThis HSP27 antibodyis to be used only for research purposes and not for diagnostics..
-
Gene ID3315
-
PurityAntigen affinity
-
DescriptionHSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. The protein encoded by this gene is induced by environmental stress and developmental changes. HSP27 is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
-
ImmunogenHuman partial recombinant protein (AA 1-205) was used as the immunogen for this HSP27 antibody.
-
StorageAfter reconstitution, the HSP27 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
-
PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolHSPB1
-
Short nameAnti-HSP27
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit IgG
-
Alternative nameAntibodies to HSP27
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameheat shock protein family B (small) member 1
-
Synonyms gene name
- heat shock 27kD protein 1
- heat shock 27kDa protein 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1991-07-09
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Small heat shock proteins
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data