PNP, human recombinant

• Catalog number
  7831-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PUNP
• Alternative_names
  Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PUNP
• Description
  Catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is > 40,000 pmol/min/µg. One unit will form 1.0 pmole of 7-Methyl-6-thioguanosine to 7-Methyl-6-thioguanine per minute at PH 7.5 at 25ºC
• Results
  Specific activity is > 40,000 pmol/min/µg
• Unit Definition
  One unit will form 1.0 pmole of 7-Methyl-6-thioguanosine to 7-Methyl-6-thioguanine per minute at PH 7.5 at 25ºC
• Molecular Weight
  34.2 kDa (309 aa, 1-289 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2 mM DTT and 0.1 M NaCl
• Background Information
  PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MENGYTYEDY KNTAEWLLSH TKHRPQVAII CGSGLGGLTD KLTQAQIFDY GEIPNFPRST VPGHAGRLVF GFLNGRACVM MQGRFHMYEG YPLWKVTFPV RVFHLLGVDT LVVTNAAGGL NPKFEVGDIM LIRDHINLPG FSGQNPLRGP NDERFGDRFP AMSDAYDRTM RQRALSTWKQ MGEQRELQEG TYVMVAGPSF ETVAECRVLQ KLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SLEKANHEEV LAAGKQAAQK LEQFVSILMA SIPLPDKAS
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PNP  
• Gene symbol
  PPY, PNP
• Short name
  PNP, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  purine nucleoside phosphorylase, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  purine nucleoside phosphorylase, NP and PRO1837 and PUNP, PNP and IDBG-2272 and ENSG00000198805 and 4860, phosphate ion binding, Cytoplasm

Gene info

• Identity
  HGNC:9327
• Gene
  PPY
• Long gene name
  pancreatic polypeptide
• Synonyms
  • PNP
• Synonyms name
  • pancreatic polypeptide Y
  • prepro-PP (prepropancreatic polypeptide)
• Locus
  17q21.31
• Discovery year
  1986-01-01
• Entrez gene record
  5539
• Pubmed identfication
  • 3753985
• RefSeq identity
  • NM_002722
• Classification
  • Neuropeptides
• VEGA ID
  OTTHUMG00000181800

Gene info

• Identity
  HGNC:7892
• Gene
  PNP
• Long gene name
  purine nucleoside phosphorylase
• Synonyms gene
  • NP
• Synonyms gene name
  • nucleoside phosphorylase
• Synonyms
  • PUNP
• Locus
  14q11.2
• Discovery year
  2001-06-22
• Entrez gene record
  4860
• Pubmed identfication
  • 6087295
• RefSeq identity
  • NM_000270.2
• VEGA ID
  OTTHUMG00000029546
• Locus Specific Databases
  • NPbase: Mutation registry for PNP deficiency
  • LRG_91

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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