Wnt-1 Recombinant Protein

• Catalog number
  40-216
• Price
  Please ask
• Size
  0.002 mg, 0.01 mg

• About recombinant protein
  Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.
• Other name of the recombinant
  INT1, OI15, BMND16, INT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog
• Recombinant in
  E. coli
• SEQUENCE
  ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL
• Activity
  The ED50 was determined by its ability to enhance BMP - 2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/mL in the presence of 200 ng/mL of human BMP - 2.
• Keep at
  The lyophilized Wnt-1 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Wnt-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Wnt-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  4885655
• Accession nr
  NP_005421.1
• Pureity
  Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  7471
• Gene name
  wingless-type MMTV integration site family, member 1
• Source
  Homo sapiens
• Uniprot
  P04628
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Wnt-1   Protein  
• Gene symbol
  WNT1
• Short name
  Wnt-1 Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Wnt-1 Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:12774
• Gene
  WNT1
• Long gene name
  Wnt family member 1
• Synonyms gene
  • INT1
• Synonyms gene name
  • wingless-type MMTV integration site family, member 1
• GenBank acession
  • X03072
• Locus
  12q13.12
• Discovery year
  2001-06-22
• Entrez gene record
  7471
• Pubmed identfication
  • 2998762
  • 3281802
• Classification
  • Wnt family
• VEGA ID
  OTTHUMG00000170403

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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