Bcl6 Antibody, ALEXA FLUOR 488
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Catalog number
bs-2734R-A488
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Price
Please ask
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Size
100ul
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Long name
Bcl6 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
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Also known as
Anti-Bcl6 PAb ALEXA FLUOR 488
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 488
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Bcl6
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Specificity
This is a highly specific antibody against Bcl6.
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Modification
Unmodified
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Modification Site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen range
520-560/706
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Subcellular location
Nucleus
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Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Bcl6
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Gene ID Number
604
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Swiss Prot
P41182
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
499nm/519nm
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Synonyms
BCL5; LAZ3; BCL6A; ZNF51; ZBTB27; B-cell lymphoma 6 protein; BCL-6; B-cell lymphoma 5 protein; BCL-5; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51; BCL6
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Bcl6 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor
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French translation
anticorps
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Gene target
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Gene symbol
BCOR, BCORP1, BCL6-AS1
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Short name
Anti-Bcl6
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 488
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Alternative name
Bcl6 Polyclonal Antibody, ALEXA FLUOR 488
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Alternative technique
antibodies
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Alternative to gene target
B-cell CLL/lymphoma 6, BCL5 and BCL6A and LAZ3 and ZBTB27 and ZNF51, BCL6 and IDBG-69349 and ENSG00000113916 and 604, metal ion binding, nuclei, Bcl6 and IDBG-149890 and ENSMUSG00000022508 and 12053, BCL6 and IDBG-634562 and ENSBTAG00000001511 and 539020
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Gene info
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Identity
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Gene
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Long gene name
BCL6 corepressor
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Synonyms gene name
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2003-05-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ankyrin repeat domain containing
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
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Identity
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Gene
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Long gene name
BCL6 antisense RNA 1
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Synonyms gene name
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Synonyms
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Locus
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Discovery year
2017-08-03
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Entrez gene record
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Pubmed identfication
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Classification
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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