Mouse Anti-Mouse Protocadherin Gamma A (pan) Monoclonal IgG2b, Clone S144-32
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Catalog numberSMC-453D-A488
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Price:
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Size100 µg
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CloneS144-32
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ImmunogenFusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma-protocadherin-A3. Human: 80% identity (68/84 amino acids identical) >75% identity with other Gamma-protocadherin-A proteins
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Antibody s full descriptionMouse Anti-Mouse Protocadherin Gamma A Monoclonal IgG2b Antibody, Clone: S144-32: ATTO 488
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Antibody s categoryMonoclonal Antibodies
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Antibody s other namePCDH gamma A Antibody, PCDH-gamma-A Antibody, PCDHGA Antibody, Protocadherin gamma A Antibody, Protocadherin gamma subfamily A Antibody, Gamma Protocadherin A (pan) Antibody, Pan-Gamma-Protocadherin-A Antibody, Pan Gamma Protocadherin A Antibody
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Raised inMouse
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Antibody s targetProtocadherin Gamma A
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Primary research fieldsNeuroscience, Cell Structure, Membrane Markers
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~100kDa. Cross-reacts with other Gamma protocadherin-A proteins. No cross-reactivity against Gamma protocadherin-B or -C proteins.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-453 was sufficient for detection of Protocadherin Gamma A (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
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Released date29-Oct-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_291064.1
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Gene number93711
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Protein numberQ91XY5
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody, Clone S144-32 (SMC-453). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody (SMC-453) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma A (pan) Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of ~100 kDa Protocadherin Gamma A (pan) protein using Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody, Clone S144-32 (SMC-453). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody (SMC-453) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa. Mouse Anti-Protocadherin Gamma A (pan) Antibody [S144-32] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-453) Mouse Anti-Protocadherin Gamma A (pan) Antibody [S144-32] used in Western Blot (WB) on Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 (SMC-453)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGKV1D-32, SNORD115-32, PPP1R1B, IGLV3-32, IGHV3-32, IGKV1-32, CUTC, ZNF138, LINC01234, LUARIS
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Short nameMouse Anti-Mouse Protocadherin Gamma A (pan) Monoclonal IgG2b, Clone S144-32
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2b, IgG2b
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LabelATTO 488
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Protocadherin g A (pan) monoclonal IgG2b, clonality S144-32
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Alternative techniquemurine, antibodies
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Clone nameS144-32
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-32 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1D-32
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-32
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 1 regulatory inhibitor subunit 1B
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Synonyms gene name
- protein phosphatase 1, regulatory (inhibitor) subunit 1B
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-32 (non-functional)
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Synonyms gene name
- immunoglobulin lambda variable 3-32
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-32 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-32
- immunoglobulin heavy variable 3-32 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-32 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1-32
- immunoglobulin kappa variable 1-32 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecutC copper transporter
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Synonyms gene name
- cutC copper transporter homolog (E. coli)
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 138
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Synonyms gene name
- zinc finger protein 138 (clone pHZ-32)
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-02-11
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Entrez gene record
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelong intergenic non-protein coding RNA 1234
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Synonyms
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Locus
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Discovery year2014-02-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long intergenic non-protein coding RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelncRNA upregulator of antiviral response interferon signaling
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Synonyms
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Synonyms name
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Locus
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Discovery year2017-03-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data