Chicken anti-Rat IgG (H&L) - Affinity Pure, HRP Conjugate
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Catalog number
MBS687663
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Price
Please ask
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Size
1 mg
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Products_type
Secondary Antibody
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Products_short_name
[IgG (H&L)]
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Products_name_syn
[Chicken anti-Rat IgG (H&L) - Affinity Pure]
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Reactivity
Rat. Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin rat serum proteins
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Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on rat IgG, light chains on all rat immunoglobulins
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Rat IgG.
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Form
Lyophilized
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Concentration
1.0 mg/ml (E 1% at 280 nm = 13.2)
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Storage_stability
Store freeze-dried powder at 2-8 degree C. Shelf Life: Store lyophilized material at 2-8 degree C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 degree C as a liquid.
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Tested_application
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
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Test
A high affinity purification column was use to purify anti- IgG (H L) - Pure, Conjugate by MyBioSource by chromatographic size exclusion.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure anti- IgG (H L) - Pure, Conjugate supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Short name
anti- IgG (H L) - Pure, Conjugate
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Technique
affinity, anti-, anti, Pure, IgG, antibody to, antibodies, IgGs, pure products
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Host
Chicken
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Isotype
IgG, Heavy & Light chains, IgG
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Label
HRP
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Species
Rat, Chickens, Rats
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Alternative name
Chicken Antibody toRat Immunoglobulin G (heavy + light chain) - protein Pure, horseradish peroxidase Conjugate
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Alternative technique
antibodies, purified, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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