Caspase-5 Substrate WEHD-AFC
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Catalog number1101-1000
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PricePlease ask
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Size1000 assays
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Alternative_nameAc-WEHD-AFC, Caspase-5 Substrate, Fluorogenic
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DescriptionReady-to-use fluorometric substrate for caspase-1,-4,-5 and related caspases that recognize the amino acid sequence WEHD. Caspase-5 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate WEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80) for caspase assays are also available separately.
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Structure AvailableNo
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Peptide sequenceAc-Trp-Glu-His-Asp-AFC
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Salt FormNo
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Molecular FormulaC₃₈H₃₇F₃N₈O₁₁
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Molecular Weight838.8
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Cell PermeableNo
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Purity>99% by HPLC
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HandlingProtect from light and moisture
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Tag LineA caspase-5 substrate
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Storage Condition-20°C
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Shipping Conditiongel pack
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Shelf Life12 months
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MDL NumberMFCD01862607
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PubChem CID44135104
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SMILESCC(=O)NC(CC1=CNC2=CC=CC=C21)C(=O)NC(CCC(=O)O)C(=O)NC(CC3=CN=CN3)C(=O)NC(CC(=O)O)C(=O)NC4=CC5=C(C=C4)C(=CC(=O)O5)C(F)(F)F
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InChiInChI=1S/C38H37F3N8O11/c1-18(50)45-27(10-19-15-43-25-5-3-2-4-22(19)25)36(58)47-26(8-9-31(51)52)34(56)48-28(11-21-16-42-17-44-21)37(59)49-29(14-32(53)54)35(57)46-20-6-7-23-24(38(39,40)41)13-33(55)60-30(23)12-20/h2-7,12-13,15-17,26-29,43H,8-11,14H2,1H3,(H,42,44)(H,45,50)(H,46,57)(H,47,58)(H,48,56)(H,49,59)(H,51,52)(H,53,54)
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InChi KeyNEGOLAGPKZGIKJ-UHFFFAOYSA-N
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MSDS AvailableYes
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Additional descriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Gene target
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Short nameCaspase-5 Substrate WEHD-AFC
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Alternative namecaspase-5 Substrate WEHD-AFC
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MeSH Data
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Name
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ConceptScope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
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