Na, Na+/H+ antiporter, sodium/hydrogen exchanger

• Catalog number
  AS09 484
• Price
  Please ask
• Size
  50 µl

• Available ordering format
  Inquire
• Immunogen
  KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NHX protein UniProt: Q68KI4, TAIR: At5g27150; chosen peptide is perfectly confirmed in AtNHX1 UniProt: Q0WVZ5, partially in AtNHX2, UniProt: Q56XP4, and not conserved in AtNHX3, UniProt: Q84WG1 and AtNHX4, UniProt:Q8S397 isoforms
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
• Verified applications
  ELISA (ELISA), western blot (WB)
• Connected products
  collection of antibodies to tonoplast proteins Plant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 1000 with standard ECL (WB), 1: 8000 (ELISA)
• Molecular weight expected аpparent
  59.5 | 45 kDa
• Verified reactivity
  Arabidopsis thaliana, Kandelia obovata
• Possible reactivity
  dictos including: Gossypium hirsutum, Ricinus communis, Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Populus euphratica
• No reactivity
  mangrove plants, sp. Avicennia, Nicotiana benthamiana
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  Chen et al. (2013). Nitric Oxide Mediates Root K+/Na+ Balance in a Mangrove Plant, Kandelia obovata, by Enhancing the Expression of AKT1-Type K+ Channel and Na+/H+ Antiporter under High Salinity. 8(8): e71543. doi:10.1371/journal.pone.0071543.
• Scientific context
  Na(+)/H(+) exchanger 1 protein is involved in exchange of protons for cations such as sodium and potassium across membraes. Localized in tonoplast, possibily also to ER and Golgi. Alternative name: NHE-1, Na(+)/H(+) exchanger 1
• Notes
  Protocol for vacuolar membrane isolation can be found here.
• Protein number
  Q68KI4
• TAIR number
  AT5G27150
• Hyperlink

• Gene target
  Na+/H   antiporter   sodium/hydrogen   exchanger  
• Gene symbol
  SLC9A1
• Short name
  Na, Na+/H+ antiporter, sodium/hydrogen exchanger
• Alternative name
  Na, Na+/H+ antiporter, sodium/hydrogen exchanger

Gene info

• Identity
  HGNC:11071
• Gene
  SLC9A1
• Long gene name
  solute carrier family 9 member A1
• Synonyms gene
  • APNH
  • NHE1
• Synonyms gene name
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive)
  • solute carrier family 9 (sodium/hydrogen exchanger), member 1
  • solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
• Synonyms
  • PPP1R143
• Synonyms name
  • protein phosphatase 1, regulatory subunit 143
• GenBank acession
  • M81768
• Locus
  1p36.11
• Discovery year
  1986-01-01
• Entrez gene record
  6548
• Pubmed identfication
  • 8283968
• RefSeq identity
  • NM_003047
• Classification
  • Solute carriers
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000004271

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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