Lipid Extraction Kit (Chloroform Free)

• Catalog number
  K216-50
• Price
  Please ask
• Size
  50 assays

• Description
  A chloroform free method of lipid isolation by organic extraction in a phase-free manner.
• Summary
  Phase free, chloroform free method of lipid isolation. Use with multiple downstream assays
• Detection Method
  N/A
• Species Reactivity
  Mammalian
• Applications
  • Lipid Extraction for downstream lipid analysis using Assay Kits (e.g. Cholesterol, Triglyceride etc.). • Lipid Extraction for downstream lipid analysis with HPLC, MS and Lipidomics. • Lipid Extraction for Screening, characterization and analysis of lipids under normal and disease conditions.
• Sample Type
  Serum, Plasma, Cultured Cells or Tissues.
• Features Benefits
  Avoids the use of chloroform - therefore non-hazardous protocol. Phase free lipid extraction - therefore minimized contamination Use with numerous downstream applications
• Storage Conditions
  RT
• Shipping Conditions
  RT
• Shelf life
  12 months
• Background
  Lipids are a group of naturally occurring molecules that include monoglycerides, diglycerides, trigylcerides, fats, sterols and others. Lipids define and preserve cellular membrane integrity and play an important role in cellular processes such as membrane trafficking, signal transduction and energy storage. Dysregulation of lipid metabolism is linked to many diseases like cancer, diabetes and heart disease. Traditionally, the Folch method of organic extraction of lipids is preferred, in which lipids are extracted into a bottom organic phase. Isolating the lipids from this lower fraction is tricky and can in part cause isolation of other phases as well. This leads to low purity of lipid samples which might negatively affect or hinder downstream lipid analysis. In addition, this method uses chloroform as the organic phase solvent, which is toxic and long-term exposure to it is hazardous. BioVision’s Lipid Extraction Kit provides a chloroform free protocol of lipid isolation by organic extraction in a phase-free manner. The recovered organic phase can be dried and resuspended for further downstream lipid analysis. This protocol not only overcomes the difficulty of lower phase extraction and the dangers of using chloroform, but also provides for a very efficient and clean lipid extraction. Each kit provides sufficient reagents to isolate up to 50 lipid preparations based on a 25 µl sample size.
• Data Sheet Link

• Gene target
  Lipid   Extraction   Kit   Chloroform   Free  
• Short name
  Lipid Extraction Kit (Chloroform Free)
• Alternative name
  Lipid Extraction reagent (Chloroform Free)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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