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Also known as
Actin, pan
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Other names
actin 5C, isoform C; Actin-5C; CG4027 gene product from transcript CG4027-RC; 5C actin; Act5C-PA; Act5C-PB; Act5C-PC; Act5C-PD; CG4027-PA; CG4027-PB; CG4027-PC; CG4027-PD; Dm-actin5C; F-actin; G-actin; actin 5 C; actin 5C; actin 5c; actin A1; anon-fast-evolving-2D2; cellular cytoskeletal beta-actin; Actin 5C; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
Act5C; Act5C; A; Ac5C; act; Act; act 5C; Act-5C; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; cyt5C; DmelCG4027; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0; N/A
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Gene name synonims
Act5C; Act5C; A; Ac5C; act; Act; act 5C; Act-5C; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; cyt5C; DmelCG4027; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0; N/A
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Other gene names
Act5C; Act5C; A; Ac5C; act; Act; act 5C; Act-5C; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; cyt5C; DmelCG4027; l(1)G0009; l(1)G0010; l(1)G0025; l(1)G0079; l(1)G0117; l(1)G0; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1,k
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Clone
3F12
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Host organism
Mouse (Mus musculus)
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Source organism
Chicken
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Species reactivity
Bovine, Canine, Chicken, Human (Homo sapiens), Mouse (Mus musculus), Porcine, Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes all six known vertebrate isoforms of actin (MW~42kD) and two cytoplasmic actins (beta, gamma) which are highly homologous to one another but which differ from the muscle actins at about 25aa. A0760-51 is highly recommended for monitoring total protein load on Western blots. Species Crossreactivity: Human, bovine, mouse, canine, porcine, rabbit, rat, chicken, Dictyostelium discoideum, Physarum polycephalum.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein G affinity chromatography.
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Form Appearance
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide before the addition of glycerol to 40%. Also available without BSA and azide. See A0760-51X
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Long-term storage should be done after aliquoting the antibody and freezing the aliqotes at -20 degrees Celsius. Under these conditions the aliquots will be stable for at least one year if kept at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
