Recombinant Human PAF-AH subunit β/PAFAHB (C-6His)

Alternative names

Platelet-Activating Factor Acetylhydrolase IB Subunit Beta, PAF Acetylhydrolase 30 kDa Subunit, PAF-AH 30 kDa Subunit, PAF-AH Subunit Beta, PAFAH Subunit Beta, PAFAH1B2, PAFAHB
Description

Recombinant Human PAF-AH subunit beta is produced by our E.coli expression system and the target gene encoding Ser2-Ala229 is expressed with a 6His tag at the C-terminus.
Expression

E. coli
Tag

C-His
Endotoxin

Less than 0.1 ng/ug (1 EU/ug)
Accession

P68402
TargetSequence

Ser2-Ala229
Shipping

Dry ice/Polar packs
Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
Note

For research use only.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

PAF-AH subunit β/PAFAHB C-6His
Gene symbol

PEX6, PLA2G7, PAFAH1B1, PEX2, PCLAF
Short name

Recombinant PAF-AH subunit β/PAFAHB (C-6His)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Bon Opus advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Species

Human, Humans
Alternative name

Rec. H. sapiens PAF-AH functionnal sequence β/PAFAHB (C-6His)
Alternative technique

rec

Identity

HGNC:9040
Gene

PLA2G7
Long gene name

phospholipase A2 group VII
Synonyms gene name
- phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Synonyms
- PAFAH
- LDL-PLA2
GenBank acession
- U20157
Locus

6p12.3
Discovery year

1999-09-20
Entrez gene record

7941
Pubmed identfication
- 7700381
- 8624782
Classification
- Phospholipases
VEGA ID

OTTHUMG00000014789

Identity

HGNC:8574
Gene

PAFAH1B1
Long gene name

platelet activating factor acetylhydrolase 1b regulatory subunit 1
Synonyms gene
- MDCR
- MDS
Synonyms gene name
- platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD)
- Miller-Dieker syndrome chromosome region
- platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
- platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)
- platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
Synonyms
- LIS1
- PAFAH
- NudF
Synonyms name
- lissencephaly-1
GenBank acession
- L25107
Locus

17p13.3
Discovery year

1998-04-03
Entrez gene record

5048
Pubmed identfication
- 8355785
- 9063735
RefSeq identity
- NM_000430
Classification
- WD repeat domain containing
VEGA ID

OTTHUMG00000177574

Identity

HGNC:9717
Gene

PEX2
Long gene name

peroxisomal biogenesis factor 2
Synonyms gene
- PXMP3
Synonyms gene name
- peroxisomal membrane protein 3 (35kD, Zellweger syndrome)
- peroxisomal membrane protein 3, 35kDa
Synonyms
- PMP35
- PAF-1
- RNF72
- ZWS3
Synonyms name
- Zellweger syndrome
- peroxin 2
GenBank acession
- M86852
Locus

8q21.13
Discovery year

1992-03-03
Entrez gene record

5828
Pubmed identfication
- 1546315
- 8858157
RefSeq identity
- NM_000318
Classification
- Peroxins
- Ring finger proteins
VEGA ID

OTTHUMG00000164530
Locus Specific Databases
- dbPEX, PEX Gene Database

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data