RAF1, Active

Synonyms

RAF, RAF proto-oncogene serine/threonine-protein kinase
Alternative_names

RAF, RAF proto-oncogene serine/threonine-protein kinase , Proto-oncogene c-RAF
Description

A proto-oncogene
Recombinant

Yes
Source

Baculovirus (Sf9 insect cells)
Purity by SDS PAGE

≥85%
Assay

SDS-PAGE
Activity Specifications test method

The specific activity of RAF1(EE) was determined to be ~6,000 nmol /min/mg in a coupled assay as per activity assay protocol.
Molecular Weight

63.0 kDa
Storage Temp

-80°C
Shipping

dry ice
Shelf Life

12 months
Concentration

5µg/50 µl
Appearance

Liquid
Physical form description

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Background Information

RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet

Gene target

RAF1 Active
Gene symbol

RNASE3, RAF1
Short name

RAF1, Active
Alternative name

v-raf-1 murine leukemia viral oncogene homolog 1, functionnal
Alternative to gene target

v-raf-1 murine leukemia viral oncogene homolog 1, c-Raf and CMD1NN and CRAF and NS5 and Raf-1, RAF1 and IDBG-19277 and ENSG00000132155 and 102724915,5894, transferase activity, nuclei, Raf1 and IDBG-179428 and ENSMUSG00000000441 and 110157, RAF1 and IDBG-646381 and ENSBTAG00000004514 and 521196