COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE), Negative Control
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Catalog number
GIC-201
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Price
Please ask
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Size
50Tests
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Host Source
Mouse
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Clone Name
VI-AP and VI-AP
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Product Category
NA
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Species Reactivity
NA
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Application
Flow cytometry
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Immunogen
NA
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Purification Method
NA
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Shipping Conditions
NA
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Storage conditions
COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE), Negative Control MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8ÁC (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended. _x000B_
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Datasheet link
NA
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
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Properties
This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short name
COMBI IC Reagent: IgG Negative Control (FITC) IgG Negative Control (PE), Negative Control
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Technique
Negative, Control, FITC, IgG, controls, Fluorescein, IgGs, negatif
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Isotype
IgG1, IgG
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Label
FITC
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Alternative name
COMBI IC Reagent: Immunoglobulin G Negative reference (fluorecein) and Immunoglobulin G Negative reference (peroxidase), Negative reference
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Alternative technique
controls, fluorescine, immunoglobulins
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Tissue
control
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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