14-3-3 sigma Antibody, ALEXA FLUOR 488
-
Catalog numberbs-12422R-A488
-
PricePlease ask
-
Size100ul
-
-
Long name14-3-3 sigma Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
-
Also known asAnti-14-3-3 sigma PAb ALEXA FLUOR 488
-
CategoryConjugated Primary Antibodies
-
Conjugated withALEXA FLUOR® 488
-
Host OrganismRabbit (Oryctolagus cuniculus)
-
Target Antigen14-3-3 sigma
-
SpecificityThis is a highly specific antibody against 14-3-3 sigma.
-
ModificationUnmodified
-
Modification SiteNone
-
ClonalityPolyclonal
-
ClonePolyclonal antibody
-
Concentration1ug per 1ul
-
SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human 14-3-3 sigma
-
Gene ID Number2810
-
Tested applicationsIF(IHC-P)
-
Recommended dilutionsIF(IHC-P)(1:50-200)
-
CrossreactivityHuman, Mouse, Rat
-
Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Background of the antigen14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events.
-
PurificationPurified by Protein A.
-
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
-
Excitation emission499nm/519nm
-
Synonyms14 3 3 protein sigma; 14-3-3 protein sigma; 1433S_HUMAN; Epithelial cell marker protein 1; Er; HME 1; HME1; MGC143283; Mkrn3; Mme1; OTTHUMP00000004242; RP23 137L22.11; SFN; SFN protein; Stratin; YWHAS.
-
PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-14-3-3 sigma has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
ConjugationAlexa Fluor
-
French translationanticorps
-
Gene target
-
Gene symbolYWHAQ, YWHAG, YWHAZ, MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12
-
Short nameAnti-14-3-3 sigma
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeImmunoglobulin G (IgG)
-
LabelALEXA FLUOR 488
-
Alternative name14-3-3 sigma Polyclonal Antibody, ALEXA FLUOR 488
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
-
Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
- protein, theta
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1999-08-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
-
Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1993-09-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
-
Synonyms gene
-
Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1993-09-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1302-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2008-11-03
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1302 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 509-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2007-10-24
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR506 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 7-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR7 family
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 14
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 12
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
