Mouse Anti-Human LRRK2/Dardarin Monoclonal IgG2a, Clone S231B-34
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Catalog numberSMC-445D-A655
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Price:
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Size100 µg
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CloneS231B-34
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ImmunogenFusion protein amino acids 841-960 of human LRRK2. 81% identical in mouse, 80% identical in rat.
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Antibody s full descriptionMouse Anti-Human LRRK2 Monoclonal IgG2a Antibody, Clone: S231B-34: ATTO 655
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameLeucine-rich repeat kinase 2 Antibody, RIP7 Antibody, PARK8 Antibody, Dardarin Antibody, ROCO 2 Antibody, RIPK7 Antibody, Leucine-rich repeat serine/threonine-protein kinase 2 Antibody, augmented in rheumatoid arthritis 17 Antibody
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Raised inMouse
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Antibody s targetLRRK2
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Primary research fieldsNeuroscience, Neurodegeneration, Parkinson's Disease, Cell Signaling, Post-Translational Modification, Phosphorylation
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Rat
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Antibody s dilutionsWB (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects >200kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis10 µg/ml of SMC-445 was sufficient for detection of LRRK2/Dardarin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Membrane , Mitochondrion , Golgi Apparatus , Cell Projection , Axon , Dendrite , Endoplasmic Reticulum , Cytoplasmic Vesicle , Endosome , Lysosome
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextLRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features.
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Released date26-Sep-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_940980
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Gene number120892
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Protein numberQ5S007
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S231B-34 (SMC-445). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (SMC-445) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LRRK2/Dardarin Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of >200 kDa LRRK2/Dardarin protein using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S231B-34 (SMC-445). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody (SMC-445) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: >200 kDa. Mouse Anti-LRRK2/Dardarin Antibody [S231B-34] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-445) Mouse Anti-LRRK2/Dardarin Antibody [S231B-34] used in Western Blot (WB) on Rat Brain Membrane (SMC-445)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD115-34, LRRK2-DT, IGKV3D-34, IGHV4-34, IGKV3-34, IGLV2-34, IGHV7-34-1, RNU6-34P, RASSF2, LRRK2
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Short nameMouse Anti- LRRK2/Dardarin Monoclonal IgG2a, Clone S231B-34
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2a, IgG2a
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LabelATTO 655
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman leucine-rich repeat kinase 2/Dardarin monoclonal IgG2a, clonality S231B-34
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Alternative techniquemurine, antibodies
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Alternative to gene targetleucine-rich repeat kinase 2, LRRK2 and IDBG-27257 and ENSG00000188906 and 120892, transferase activity, Extracellular, Lrrk2 and IDBG-175625 and ENSMUSG00000036273 and 66725, LRRK2 and IDBG-633456 and ENSBTAG00000016260 and 535652
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Clone nameS231B-34
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-34
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameLRRK2 divergent transcript
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Locus
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Discovery year2019-08-16
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Entrez gene record
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Classification
- Divergent transcripts
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3D-34 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3D-34
- immunoglobulin kappa variable 3D-34 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 4-34
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3-34 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3-34
- immunoglobulin kappa variable 3-34 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 2-34 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 2-34
- immunoglobulin lambda variable 2-34 pseudogene
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 7-34-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 7-34-1
- immunoglobulin heavy variable 7-34-1 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 34, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 34
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameRas association domain family member 2
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Synonyms gene name
- Ras association (RalGDS/AF-6) domain family member 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ras association domain family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameleucine rich repeat kinase 2
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Synonyms gene
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Synonyms gene name
- Parkinson disease (autosomal dominant) 8
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-10-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
- ROCO family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data