Procathepsin K, rat recombinant

• Catalog number
  1029-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  CTSK, CTSO, CTSO3
• Alternative_names
  CTSK, CTSO, CTSO3
• Description
  Predominant proteinase responsible for the resorption of the bone matrix.
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  >400 mU/mg
• Biological activity
  The specific activity is >400 mU/mg (1U = 1 µmole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog # K141-100)
• Results
  >400 mU/mg
• Unit Definition
  1 U = 1 µmole/min/mg
• Molecular Weight
  35.4 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0).
• Background Information
  Cathepsin K, a member of the papain cysteine proteinase family, is the predominant proteinase responsible for the resorption of the bone matrix. Cathepsin cleaves proteins such as collagen type I, collagen type II and osteonectin, thereby playing a role in bone remodeling and resorption in osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is produced as an inactive proenzyme (35.4 kDa) that is converted to its mature active form (23.5 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Procathepsin  
• Short name
  Procathepsin K, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  Procathepsin K, rat Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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