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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
chromatin immunoprecipiation (ChIP), immunofluorescence (IF), immunohistochemistry (IHC), western blot (WB)
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Connected products
AS16 3175 | anti-H3T6pK9me2 | Histone H3 (dimethyl Lys9, p Thr6) , rabbit antibodies AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies AS16 3169 | anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodiesAS16 3197 | anti-anti-H3core, monoclonal antibodiesAS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodiesAS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodiesAS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710collection of antibodies to epigenetics
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Recommended dilutions for use
chromatin immunoprecipiation (ChIP), immunofluorescence (IF), immunohistochemistry (IHC), western blot (WB)
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Molecular weight expected аpparent
15 kDa
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Verified reactivity
C.elegans, human
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Possible reactivity
chicken, D. melanogaster, mouse, plant, rat, Xenopus sp.
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No reactivity
No confirmed exceptions from predicted reactivity known at the moment
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Supplementary information
To be added when available
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References
To be added when available, antibody released in February 2016.
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Scientific context
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimethylation of H3 Arg8 (H3 R8Me2) is known as a mark of transcriptional repression.
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Notes
This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
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Protein number
Q71DI3
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TAIR number
Refer to NCBI
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