Annexin V-Cy5 Apoptosis Kit

Description

Detects Apoptosis within 10 min.
Summary

• Detection method- Flow cytometry (Ex = 649 nm; Em = 670 nm) and fluorescence microscopy • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 400 assays)
Sample Type

Living cells (suspension and adherent)
Features Benefits

• Simple one step staining procedure in 10 minutes • Fast and convenient • Annexin V-Cy5 will show bright red-blue staining on the plasma membrane
Storage Conditions

+4°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy.
Data Sheet Link
Additional description

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies. This 1 is suited for programmed cell-death studies.
Properties

Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
Conjugation

Cy5.5

Gene target

Annexin Kit
Short name

Annexin V- Apoptosis Kit
Technique

apoptosis, apoptosis pathway
Label

Cy5
Alternative name

Annexin V-cyanine 5 programmed cellular destruction reagent
Alternative technique

celldeath, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832