Rabbit Anti-Human LATS1 (pSer909) Polyclonal: RPE
-
Catalog numberSPC-1005D-RPE
-
PricePlease ask
-
Size100 µl
-
-
ImmunogenA phospho-specific peptide corresponding to residues surrounding Ser909 of human LATS1 (AA906-912)
-
Antibody s targetHuman LATS1 (pSer909)
-
Antibody s full descriptionRabbit Anti-Human LATS1 (pSer909) Polyclonal
-
Antibody s categoryPolyclonal Antibodies
-
Antibody s other nameh-warts Antibody, Large tumor suppressor homolog 1 Antibody, LATS large tumor suppressor homolog 1 Antibody, LATS1 Antibody, Serine threonine protein kinase LATS1 Antibody, WARTS Antibody, WARTS protein kinase Antibody, wts Antibody
-
Verified applicationsWB, AM
-
Raised inRabbit
-
Antibody s reactivityHuman
-
Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
-
Antibody s purified fromPeptide Affinity Purified
-
Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
-
Antibody s concentrationSee included datasheet or contact our support service
-
Antibody s specificityDetects 126.87 kDa.
-
Storage recommendations-20°C
-
Shipping recommendationsBlue Ice or 4°C
-
Certificate of analysisA 1:250 dilution of SPC-1005 was sufficient for detection of LATS1 (pSer909) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cellCytoplasm, Cytoskeleton
-
Tissue specificityExpressed in all adult tissues examined except for lung and kidney.
-
Scientific contextLATS1 is a protein serine/threonine kinase that suppresses tumour progression by restricting proliferation and promoting apoptosis by activating the transcription of BAX, caspase 3 and p53. It can phosphorylate and inactivate the YAP1 oncoprotein and inhibit its translocation to the nucleus for transcription of genes important for proliferation and migration. LATS1 also negatively regulates the G2/M transition by downregulating CDK1 kinase activity. M669I and R806P mutations have been associated with lung adenocarcinoma and lung large cell carcinoma, respectively. Decreased LATS1 expression due to hypermethylation of the promoter have been associated with breast cancer, acute lymphoblastic leukemia, ovarian cancer, and astrocytoma. However, the very low frequency of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours.
-
BibliographyRefer to PubMed
-
Released date29/Aug/2016
-
NCBI numberNP_004681
-
Gene number9113
-
Protein numberO95835
-
PubMed numberRefer to PubMed
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
-
Representative figure linkNo Data Available
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilitIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
TestStressMark antibodies supplies antibodies that are for research of human proteins.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
GroupPolyclonals and antibodies
-
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
Gene target
-
Gene symbolRPE, LATS1
-
Short nameRabbit Anti- LATS1 (pSer909) Polyclonal: RPE
-
Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
HostRabbit, Rabbits
-
IsotypeImmunoglobulin
-
LabelRPE
-
SpeciesHuman, Humans
-
Alternative nameproduction species: rabbit Antibody toHuman LATS1 (pSer909) polyclonal: RPE
-
Alternative techniquepolyclonals, rabbit-anti, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameribulose-5-phosphate-3-epimerase
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namelarge tumor suppressor kinase 1
-
Synonyms gene name
- LATS (large tumor suppressor, Drosophila) homolog 1
- LATS, large tumor suppressor, homolog 1 (Drosophila)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1999-02-18
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- AGC family kinases
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data