Rat Anti-Mouse CD11a/LFA-1a-UNLB
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Catalog number
GEN673199
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Price
Please ask
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Size
500ug
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Also known as
CD11a (LFA-1 alpha)
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Other names
N/A
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Category
Secondary Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
Rat (Lewis) IgG2a kappa
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Clone
I21/7
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Host organism
Rat (Rattus norvegicus)
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
alpha subunit (Mr 180 kDa) of LFA-1 The monoclonal antibody I21/7 reacts with the alpha subunit of mouse CD11a, also known as Leukocyte Function-associated Antigen-1 (LFA-1) and a member of the integrin family of cell adhesion molecules.^1, 2 The antigen is expressed on thymocytes, T and B lymphocytes, and on non-lymphoid cells in the bone marrow. ^2 CD11a appears to be an essential component for T-cell activation by antigen-presenting cells and is important for cell-mediated killing. Co-stimulatory signaling by LFA-1 is achieved by pairing with its ligand, CD54/ICAM-1. ^1, 2; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Purified (UNLB) Antibody
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Concentration
N/A
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Storage and shipping
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. The R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product the antibody should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested applications
Flow Cytometry; Immunoblot; Immunohistochemistry (Frozen Sections); Immunoprecipitation
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbol
ITGAL, ITGB2
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Short name
Anti-Mouse CD11a/LFA-1a-UNLB
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Technique
anti-, Mouse, anti, antibody to, antibodies, mouses
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Host
Rat
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Species
Mouse, Mouses, Rats
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Alternative name
Anti-Rat Anti-Mouse CD11a/LFA-1a-UNLB
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Alternative technique
murine, antibodies
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Gene info
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Identity
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Gene
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Long gene name
integrin subunit alpha L
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Synonyms gene
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Synonyms gene name
- integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
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Synonyms
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Synonyms name
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Integrin alpha subunits
- CD molecules
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
integrin subunit beta 2
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Synonyms gene
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Synonyms gene name
- integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit)
- integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- Integrin beta subunits
- Complement system regulators and receptors
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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