UltraPolymer Donkey Anti-Sheep IgG (H&L) HRP

• Catalog number
  A1279-15
• Price
  Please ask
• Size
  15 ml

• Antibody Target
  Sheep IgG
• Target Alternative Name
  Anti-sheep IgG, HRP-Linked Antibody, HRP linked secondary antibody
• Antibody Type
  Secondary antibody
• Species Reactivities
  Sheep
• Immunogen Sequence
  Sheep IgG (H&L)
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS with 1% BSA and 0.1% proclin 150
• Purification
  Affinity Purified
• Application
  IHC
• Application And Usages
  IHC: 120-150 µl
• Description
  UltraPolymer Donkey anti-Sheep IgG (H&L) conjugated to HRP, affinity purified, minimum crossreactivity with human, mouse or rabbit IgG
• Storage Condition
  4 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Latin name
  Equus africanus asinus
• Properties
  HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  HRP Conjugated
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Gene target
  UltraPolymer  
• Short name
  UltraPolymer Anti- IgG (H L)
• Technique
  anti-, anti, IgG, antibody to, antibodies, IgGs
• Host
  Sheep, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
• Isotype
  Donkey IgG, Heavy & Light chains, IgG
• Label
  HRP
• Species
  Sheep, Donkeys, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  UltraPolymer Donkey Antibody toSheep Immunoglobulin G (heavy + light chain) horseradish peroxidase
• Alternative technique
  antibodies, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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