Mouse Monoclonal IgG1/k Anti-GATA3 (L50-823) [L50-823]
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Catalog numberGAT001-0.5
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Price:
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Size0.5 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenConserved peptide between the GATA trans-activation and DNA-binding domain
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SpecificityGATA3; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3
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Species reactivityHuman, rat, others not tested
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Product presentationPurified antibody in PBS with 0.2% BSA and 15 mM sodium azide
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG1/k
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Antibody s cloneL50-823
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Product is supplied asconcentrate
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Recommended concentration for use1:50-1:500
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Protein concentrationN/A
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Positive controlBreast carcinoma, Urothelial carcinoma (nuclear staining)
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PretreatmentHIER (heat induced epitope retrieval) using BioLogo's Unmasking Fluid T (TEC-Buffer, pH 8, Art. No. DE005).
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Tested applicationsIHC (P)
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Recommended secondary reagentWe recommend the use of our Universal Staining Systems AEC or DAB (Art. No. AE005 and DA005) as well as ABC- or Polymer Systems containing anti-mouse IgG conjugates.
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Storage methodStore at 2-8°C
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UniProt numberP23771 (GATA3_HUMAN)
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Litterature1. Higgins JP et al. Placental S100 (S100P) and GATA3: markers for transitional epithelium and urothelial carcinoma discovered by complementary DNA mircoarray. Am J Surg Pathol. 2007; 31:673-680. 2. Liu H et al. Immunohistochemical evaluation of GATA3 expression in tumors and normal tissues. Am J Clin Pathol. 2012; 138:57-64. 3. Miettinen M et al. GATA 3 – A multispecific but potentially useful marker in surgical pathology – a systematic analysis of 2500 epithelial and non-epithelial tumors. Am J Surg Pathol. 2014; 38:13-22.
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolMRPL50, GATA3, GATA3-AS1
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Short nameResearch
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TechniqueMouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG1
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SpeciesMouse, Mouses
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Alternative nameGATA3 (L50-823)
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene namemitochondrial ribosomal protein L50
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-10-12
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Entrez gene record
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RefSeq identity
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Classification
- Mitochondrial ribosomal proteins
- Large subunit mitochondrial ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameGATA binding protein 3
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Synonyms gene name
- GATA-binding protein 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-11-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- GATA zinc finger domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameGATA3 antisense RNA 1
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GenBank acession
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Locus
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Discovery year2012-12-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data