Crystal Violet Cell Cytotoxicity Assay Kit
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Catalog number
K329-1000
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Price
Please ask
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Size
1000 assays
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Summary
• Detection method- Absorbance (570 nm) • Sample type- Cell culture (adherent and non adherent) • Species reactivity- Mammalian • Applications- This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of crystal violet to bind ribose molecules (i.e. DNA) in viable cells.
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Detection Method
Detection method- Absorbance (570 nm)
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Species Reactivity
Species reactivity- Mammalian
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Applications
This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays.This assay relies on the ability of crystal violet to bind ribose molecules (i.e. DNA) in viable cells.
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Sample Type
Cell cultures (adherent and non-adherent)
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Features Benefits
• Simple procedure; takes less than 2 hours • Fast, sensitive and convenient
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Crystal violet (CV) cell cytotoxicity assay is one of the common methods used to detect cell viability or drug cytotoxicity. CV is a triarylmethane dye that can bind to ribose type molecules such as DNA in nuclei. Normally, dead adherent cells will detach from cell culture plates and will be removed from viable cell propulation during washing steps. CV staining can be used to quantify the total DNA of the remaining population and thus determine cell viability. The CV staining is directly proportional to the cell biomass and can be measured at 570 nm. CV staining is a quick and versatile assay for screening cell viability under diverse stimulation or inhibition conditions. BioVision’s CV cell cytotoxicity assay kit is simple, accurate, reproducible and sensitive. It also includes Doxorubicin as a positive control. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening
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Test
Biovision supplies other types of Assays as 1.
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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Gene target
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Short name
Crystal Violet Cytotoxicity Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
Crystal Violet cellular Cytotoxicity test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell
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MeSH Data
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Name
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Concept
Scope note:
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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