pCMV-Flag-PSMB4 Plasmid

• Catalog number
  PVTB00753-2a
• Price
  380.85 USD
• Size
  2 ug

• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag
• Kit
  Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

• Gene target
  pCMV   PSMB4  
• Gene symbol
  PSMB4
• Short name
  pCMV- -PSMB4 Plasmid
• Technique
  plasmid, plasmids in 1
• Label
  Flag
• Alternative name
  pCMV-Flag-proteasome (prosome, macropain) subunit, b classification, 4 Plasmid
• Alternative technique
  plasmids
• Alternative to gene target
  proteasome (prosome, macropain) subunit, beta type, 4, HN3 and HsN3 and PROS-26 and PROS26, PSMB4 and IDBG-102368 and ENSG00000159377 and 5692, threonine-type endopeptidase activity, nuclei, Psmb4 and IDBG-170872 and ENSMUSG00000005779 and 19172, PSMB4 and IDBG-633103 and ENSBTAG00000021288 and 506203

Gene info

• Identity
  HGNC:9541
• Gene
  PSMB4
• Long gene name
  proteasome 20S subunit beta 4
• Synonyms gene name
  • proteasome (prosome, macropain) subunit, beta type, 4
  • proteasome subunit beta 4
• Synonyms
  • HN3
  • PROS26
• Synonyms name
  • proteasome subunit β7
• GenBank acession
  • D26600
• Locus
  1q21.3
• Discovery year
  1995-05-03
• Entrez gene record
  5692
• Pubmed identfication
  • 7918633
• RefSeq identity
  • NM_002796
• Classification
  • Proteasome
• VEGA ID
  OTTHUMG00000012494

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