ASK1/MAPKKK5 Antibody
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Catalog number3128-100
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PricePlease ask
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Size100 µg
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Antibody TargetASK1/MAPKKK5
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Target Alternative NameMAP3K5, MEKK5, ASK-1 , MAPKKK5
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceSynthetic peptide corresponding to residue surrounding amino acid 1359 of human ASK1
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AccessionQ99683
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Gene Id4217
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AppearanceColorless liquid
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FormLiquid
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Concentration0.2 mg/ml
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Formulation100 µg (0.2 mg/ml) immunoaffinity purified, rabbit anti-ASK1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
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PurificationAffinity purified
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ApplicationWestern blot, Immunofluorescence
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Application And UsagesWestern blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects the 155 kDa ASK1 protein in human SW1353 cell lysate by immunoblotting.
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DescriptionASK1 (Apoptosis Signal-regulating Kinase 1) activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N-terminal Kinase (JNK) and p38 MAP Kinase. ASK1 is activated by TNFR and Fas throμgh the interaction with members of the TRAF Family and the Fas-associated protein Daxx. Overexpression of ASK1 induces apoptosis. However, a catalytically inactive form of ASK1 has been shown to inhibit TNF-α induced apoptosis.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMAP3K5
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Short nameASK1/MAPKKK5 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameASK1/MAPKKK5 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namemitogen-activated protein kinase kinase kinase 5
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-11-14
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Entrez gene record
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Pubmed identfication
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Classification
- Mitogen-activated protein kinase kinase kinases
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data