HSF1, Heat shock factor 1

  • Catalog number
    AS06 178
  • Price
    Please ask
  • Size
    100 µl
  • Available ordering format
    Lyophilized
  • Immunogen
    C-terminal part of Chlamydomonas reinhardtii HSF1 (Exons 7 to 9), UniProt: A1YTN3
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Serum
  • How to reconstitute
    For reconstitution add 100 µl of sterile water.
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    collection of antibodies to other heat shock proteinscollection of antibodies to Chlamydomonas reinhardtiiAlgal protein extraction buffer Secondary antibodies
  • Recommended dilutions for use
    1. 3000 with standard ECL (WB)
  • Molecular weight expected аpparent
    78.4 | 100 kDa
  • Verified reactivity
    Chlamydomonas reinhardtii
  • Possible reactivity
    Chlamydomonas reinhardtii
  • No reactivity
    higher plants
  • Supplementary information
    unspecific cross-reactions at 40-50 kDa
  • References
    Schulz-Raffelt et al. (2007).. Heat shock factor 1 is a key regulator of the stress response in Chlamydomonas. The Plant Journal 52: 286-295
  • Scientific context
    HSF1 is a key regulator of the stress response. It becomes hyperphosphorylated following heat shock (results in the shifting of the HSF1 protein to slightly higher MW) and HSF1 protein rapidly accumulates during heat stress. HSF1 appears to be constitutively present as high molecular weight complexes, presumably trimers. It also interacts with cytosolic HSP70A.
  • Notes
    None
  • Protein number
    A1YTN3
  • TAIR number
    Refer to NCBI
  • Description
    Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
  • Gene target
    HSF1   Heat   shock   factor  
  • Gene symbol
    HSF1
  • Short name
    HSF1, Heat shock factor 1
  • Alternative name
    heat shock transcription factor 1, Heat shock factor 1
  • Alternative to gene target
    heat shock transcription factor 1, HSTF1, HSF1 and IDBG-40498 and ENSG00000185122 and 3297, sequence-specific DNA binding, nuclei, Hsf1 and IDBG-155311 and ENSMUSG00000022556 and 15499, HSF1 and IDBG-643592 and ENSBTAG00000020751 and 506235
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