Goat Anti-Rabbit IgG-Fc Antibody, FITC Conjugated
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Catalog number
MBS198080
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Price
Please ask
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Size
1 mg
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Products_type
Secondary Antibody
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Products_short_name
[IgG-Fc]
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Products_name_syn
[Anti-Rabbit IgG, Fc Specific-FITC]
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Products_gene_name
[IgG-Fc]
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Clonality
Polyclonal
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Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG-Fc. Reactivity with Fab fragments is not detected. This antibody may cross-react with IgG from other species.
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Storage_stability
Store at 2-8 degree C. Shelf life is one (1) year from date of receipt.
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Tested_application
Immunohistochemistry (IHC), Immunocytochemistry (ICC)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus, Oryctolagus cuniculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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French translation
anticorps
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Gene target
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Short name
Goat Anti-Rabbit IgG-Fc Antibody, FITC Conjugated
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Technique
Antibody, Rabbit, anti-, anti, FITC, Goat, IgG, FC, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgG, IgG
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Label
FITC
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Species
Goats
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Alternative name
caprine Antibody tohost: rabbit Immunoglobulin G-fragment c (antibody to-), fluorecein coupled
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Alternative technique
antibodies, rabbit-anti, caprine, fluorescine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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