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Available ordering format
Lyophilized
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Immunogen
15 aa peptide sequence from the C-terminus of Zea mays Hsp101 Q9S822
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized at -20°C; once reconstituted to a final volume this antibody can be kept in 4°C for up to one year, in smaller portions to avoid contamination. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS07 287 | anti-HSP101 | ClpB heat shock protein, C-terminal rabbit antibodyAS08 283 | anti-HSP101 | ClpB heat shock protein, N-terminal hen antibodyAS08 290 | anti-HSP101 | nuclear/cytoplasmatic heat shock protein rabbit antibody with reactivity to Zea mayscollection of antibodies to plant heat shock proteins
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Recommended dilutions for use
1: 2000 with standard ECL (WB)
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Molecular weight expected аpparent
101 kDa
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Verified reactivity
Zea mays
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Possible reactivity
Zea mays
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
this antibody will not recognize any other cereal hsp101
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References
Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferase induction and attenuation of Hsp gene expression during endosperm modification in Quality Protein Maize. Plant Physiol Dec. 8 (ahead of print).Nieto-Sotelo et al. (2002). Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633. Nieto-Sotelo et al. (1999). Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue. Gene 230: 187-195.
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Scientific context
Hsp101/ClpB is a member of HSP100 protein family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones.Besides expression during heat stress, members of HSP100 protein family are also expressed during seed development. Hsp101 protein is both nuclear- and cytoplasmic-localized (1, 2).
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Notes
None
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Protein number
Q9S822
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TAIR number
Refer to NCBI
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