MAPKAPK5(Thr182) Antibody, ALEXA FLUOR 555
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Catalog number
bs-5504R-A555
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Price
Please ask
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Size
100ul
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Long name
MAPKAPK5(Thr182) Polyclonal Antibody, ALEXA FLUOR 555 Conjugated
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Also known as
Anti-MAPKAPK5 Thr182 PAb ALEXA FLUOR 555
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 555
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
MAPKAPK5 Thr182
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Specificity
This is a highly specific antibody against MAPKAPK5 Thr182.
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Modification
Phosphorylation
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Modification Site
Thr182
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen range
160-210/473
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Subcellular location
Cytoplasm, Nucleus
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Source
KLH conjugated synthetic phosphopeptide derived from human MAPKAPK5 around the phosphorylation site of Thr182
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Gene ID Number
8550
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Swiss Prot
Q8IW41
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
553nm/568nm
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Synonyms
MK5; MK-5; PRAK; MAPKAP-K5; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; p38-regulated/activated protein kinase; MAPKAPK5
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Properties
For facs or microscopy Alexa 1 conjugate. Very high photo stable ALEXA conjugate. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor, ALEXA FLUOR 555
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French translation
anticorps
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Gene target
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Gene symbol
MAPKAPK5-AS1, MAPKAPK5
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Short name
Anti-MAPKAPK5 Thr182
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 555
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Alternative name
MAPKAPK5(Thr182) Polyclonal Antibody, ALEXA FLUOR 555
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
MAPKAPK5 antisense RNA 1
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Synonyms gene
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Synonyms gene name
- chromosome 12 open reading frame 47
- MAPKAPK5 antisense RNA 1 (non-protein coding)
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Synonyms
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Locus
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Discovery year
2006-01-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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