CD3 antibody FITC
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Catalog number61R-1000
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PricePlease ask
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Size100 µl
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchImmunology
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Type of ImmunogenCD3 antibody (FITC) was raised in Mouse using a purified recombinant fragment of human CD3 expressed in E. coli ..
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Raised inMouse
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Cross ReactivityHuman
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IsotypesIgG2a
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Clone4D10A6
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Conjugate TagFITC
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Concentration1.0 mg/ml
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Form BufferPurified antibody Provided in PBS containing 0.03% NaN3.
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StorageStore at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forFC; IF
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Usage RecommendationsFC: 1:100-1:500, IF: 1:100-1:500
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolCD3G, CD3E
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Short nameCD3 antibody FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameMouse monoclonal CD3 antibody (FITC)
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameCD3g molecule
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Synonyms gene name
- CD3g antigen, gamma polypeptide (TiT3 complex)
- CD3g molecule, gamma (CD3-TCR complex)
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GenBank acession
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Locus
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Discovery year1988-05-11
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameCD3e molecule
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Synonyms gene name
- CD3e antigen, epsilon polypeptide (TiT3 complex)
- CD3e molecule, epsilon (CD3-TCR complex)
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data