c-erbB2, p185 (HER2, neu Receptor) (FITC)
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Catalog number
MBS602582
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Price
Please ask
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Size
100 Tests
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Products_type
Antibody
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Products_short_name
[c-erbB2, p185]
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Products_name_syn
[Anti -c-erbB2, p185 (HER2, neu Receptor) (FITC)]
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Clonality
Monoclonal
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Clone
[2B2.147]
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Reactivity
Human
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Specificity
Recognizes human p185 HER-2.
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Purity
Highly Purified 95% prior to addition of stabilizer and preservative, as determined by SDS-PAGE.
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Form
Supplied as a liquid in 50mM Tris, 100mM sodium chloride, pH 7.4, 1% BSA, 0.02% sodium azide. Conjugated to FITC.
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Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Gene target
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Gene symbol
ERBB2
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Short name
c-erbB2, p185 (HER2, neu Receptor) (FITC)
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Technique
FITC, Fluorescein
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Host
Mouse
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Isotype
IgG1
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Label
FITC
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Alternative name
c-v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), p185 (HER2, neu Receptor) (fluorecein)
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Alternative technique
fluorescine
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Alternative to gene target
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), CD340 and HER-2 and HER-2/neu and HER2 and MLN 19 and NEU and NGL and TKR1, ERBB2 and IDBG-46068 and ENSG00000141736 and 2064, transferase activity, nuclei, Erbb2 and IDBG-210591 and ENSMUSG00000062312 and 13866, ERBB2 and IDBG-639410 and ENSBTAG00000021798 and 505709
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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