Neutrophil Definsin 5 (NP-5), natural, synthetic & recombinant, Clone: R3, Mouse Monoclonal antibody-Rabbit; frozen/paraffin, IH/flow/WB
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Catalog numberYM3046
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PricePlease ask
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Size0.1mg
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Alternative name 1Neutrophil Definsin 5 (NP-5), natural, synthetic & recombinant, Clone: R3, Mab anti-Rabbit; frozen/paraffin, IH/flow/WB
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Alternative name 2Neutrophil Definsin 5 (NP-5), natural, synthetic & recombinant, Clone: R3, Mab antibody-Rabbit; frozen/paraffin, IH/flow/WB
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Alternative name 3Neutrophil Definsin 5 (NP-5), natural, synthetic & recombinant, Clone: R3, Mab antibody-Rabbit; frozen/paraffin, IH/flow/WB
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Alternative name 4anti-Neutrophil Definsin 5 NP-5 natural synthetic & recombinant Clone: R3 /
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Alternative name 5Neutrophil Definsin 5 NP-5 natural synthetic & recombinant Clone: R3 /
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by accurate-monoclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. accurate-monoclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameMus musculus, Oryctolagus cuniculus
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SourceRecombinants or rec. proteins
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Grouprecombinants
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French translationanticorps
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Gene target
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Gene symbolCTF2P, PNP, NPC2, ZNF384, MIR1302-5, PIRC22, PIRC23, PIRC20, PIRC21, HTR6
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Short nameNeutrophil Definsin 5 (NP-5), natural, synthetic & recombinant, Clone: R3, Mouse Monoclonal antibody-Rabbit; frozen/paraffin, IH/ /
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TechniqueMouse monoclonal, Recombinant, Antibody, paraffin, frozen, Rabbit, Mouse, Flow, WB, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. accurate-monoclonals advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Western Blot on a membrane
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG. Rabbits
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SpeciesMouse, Mouses
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Alternative nameNeutrophil Definsin 5 (NP-5), natural, synthetic & Rec., clonality: R3, Mouse monoclonal (antibody to-)-production species: rabbit; -25°C frozen/paraffin, IH/flow/Western Blot
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Alternative techniquerec, antibodies, rabbit-anti, murine
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Tissuefrozen
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Gene info
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Identity
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Gene
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Long gene namecardiotrophin 2, pseudogene
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Synonyms
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Synonyms name
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Locus
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Discovery year2006-11-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukin 6 type cytokine family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepurine nucleoside phosphorylase
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Synonyms gene
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Synonyms gene name
- nucleoside phosphorylase
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameNPC intracellular cholesterol transporter 2
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Synonyms gene name
- Niemann-Pick disease, type C2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 384
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Synonyms gene
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Synonyms gene name
- trinucleotide repeat containing 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-01-27
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Entrez gene record
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Pubmed identfication
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-5
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 22
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 23
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 20
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 21
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene name5-hydroxytryptamine receptor 6
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Synonyms gene name
- 5-hydroxytryptamine (serotonin) receptor 6
- 5-hydroxytryptamine (serotonin) receptor 6, G protein-coupled
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-12-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 5-hydroxytryptamine receptors, G protein-coupled
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data