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Gene info
The p53 gene like the Rb gene, is a tumor suppressor gene, i.e., its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are pre-disposed to cancer and usually develop several independent tumors in a variety of tissues in early adulthood. This condition is rare, and is known as Li-Fraumeni syndrome. However, mutations in p53 are found in most tumor types, and so contribute to the complex network of molecular events leading to tumor formation. In the cell, p53 protein binds DNA, which in turn stimulates another gene to produce a protein called p21 that interacts with a cell division-stimulating protein (cdk2). When p21 is complexed with cdk2 the cell cannot pass through to the next stage of cell division. Mutant p53 can no longer bind DNA in an effective way, and as a consequence the p21 protein is not made available to act as the 'stop signal' for cell division. Thus cells divide uncontrollably, and form tumors. p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. The amount of information that exists on all aspects of p53 normal function and mutant expression in human cancers is now vast, reflecting its key role in the pathogenesis of human cancers. It is clear that p53 is just one component of a network of events that culminate in tumor formation.
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Gene names
TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1
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Antibodie s label
unlabelled serum
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Purity
Protein A Column
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Ab raised in
Mouse
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Type
Monoclonal
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Immunoglobulin
IgG2a,k
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Antigen
TP53 monoclonal antibody was made against amino acids 1 - 534 of TP53 (Human) using recombinant human p53 protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma P3-X63/AG8.653 cells using conventional hybridoma technology.
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Immuno application
ELISA, Western Blot, immuno-histo-chemistry, immuno precipitation
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Antibody advice
TP53 antibody can be used in ELISA starting at 1:1000, and immunohistochemistry starting at 10 ug/mL.
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Suspention pH azide
0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.
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Keep at
Store TP53 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
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Works with
Human
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Omim nr
269849759
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Ncbi nr
P04637
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Vial with antibody
in solution
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NCBI GENE nr
7157
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GENE symbol
TP53
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Antigene name
tumor protein p53
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Source
Homo sapiens
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Protein nr
P04637
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Gene symbol
TP53
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Short name
anti-TP53 Antibody, Monoclonal
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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Alternative name
Antibody toTP53 (antibody to-), monoclonal
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Alternative technique
antibodies
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Alternative to gene target
tumor protein p53, BCC7 and LFS1 and P53 and TRP53, TP53 and IDBG-26364 and ENSG00000141510 and 7157, MDM2/MDM4 family protein binding, nuclei, Trp53 and IDBG-191036 and ENSMUSG00000059552 and 22059, TP53 and IDBG-635147 and ENSBTAG00000001069 and 281542
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