Prothrombin, Human Plasma
-
Catalog number
7684-1
-
Price
Please ask
-
Size
1 mg
-
-
Synonyms
Coagulation factor II
-
Alternative_names
Coagulation factor II
-
Description
Catalyzes the coagulation of fibrinogen
-
Recombinant
No
-
Source
Human Plasma
-
Purity by SDS PAGE
≥95%
-
Assay
SDS-PAGE
-
Molecular Weight
72 kDa
-
Storage Temp
-80°C
-
Shipping
Dry Ice
-
Shelf Life
12 months
-
Appearance
Liquid
-
Physical form description
In 20 mM Tris-HCl, 0.1 M NaCl pH 7.4
-
Background Information
Prothrombin is a vitamin K-dependent plasma protein which is synthesized in the liver. Prior to secretion into plasma, prothrombin undergoes post-translational modification by a vitamin K-dependent carboxylase which converts ten specific glutamic acid residues to γ-carboxyglutamic acid (gla). Conversion to thrombin is a key step in the blood coagulation pathway and catalyzes the coagulation of fibrinogen. Clinically, cases of selective deficiency are rare, although, in cases of liver cirrhosis, prothrombin is decreased. During activation, prothrombin is cleaved at Arg271-Thr272 and at Arg320-Ser321 to a "pro" fragment (fragment 1.2) and thrombin, the latter of which is composed of two chains covalently linked by a disulfide bond. There is an additional thrombin feed-back cleavage at Arg284-Thr285 resulting in an additional 13 amino acids being removed from the mature thrombin “A” chain.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Product images
Similar products
