Biotin-NHS

Alternative_name

Biotin-N-hydroxysuccinimide ester; (+)-Biotin N-succinimidyl ester, d-Biotin NHS ester, BNHS, Biotinyl-N-hydroxy-succinimide, N-Hydroxysuccinimidobiotin
Description

Biotin-NHS is a useful reagent for biotinylation of primary amines under mild conditions (pH~6.5-8.5). Some examples of primary amines for biotinylation include lysines on the surface of proteins, antibodies, etc.
CAS Number

35013-72-0
Structure Available

Yes
Salt Form

No
Molecular Formula

C₁₄H₁₉N₃O₅S
Molecular Weight

341.38
Cell Permeable

No
Purity

≥95% by HPLC
Solubilities

DMF (~ 50 mg/ml)
Handling

Protect from air and moisture
Tag Line

A biotinylation agent for primary amines
Storage Condition

-20 ̊C
Shipping Condition

gel pack
Shelf Life

36 months
MDL Number

MFCD00078531
PubChem CID

434213
PubChem CID Link
SMILES

C1CC(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3
InChi

InChI=1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)
InChi Key

YMXHPSHLTSZXKH-UHFFFAOYSA-N
Data Sheet Link
MSDS Available

Yes
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated

Identity

HGNC:7820
Gene

NHS
Long gene name

NHS actin remodeling regulator
Synonyms gene name
- Nance-Horan syndrome (congenital cataracts and dental anomalies)
Locus

Xp22.2-p22.13
Discovery year

1989-06-30
Entrez gene record

4810
Pubmed identfication
- 20332100
RefSeq identity
- NM_198270
Classification
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000022799
Locus Specific Databases
- Global Variome shared LOVD