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Also known as
Microphthalmia
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Other names
microphthalmia associated transcription factor; Microphthalmia-associated transcription factor; microphthalmia-associated transcription factor; microphthalmia-associated transcription factor; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
Mitf; Mitf; N/A; N/A
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Gene name synonims
Mitf; Mitf; N/A; N/A
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Other gene names
Mitf; Mitf; N/A; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1,k
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Clone
3F275
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Canine, Equine, Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes human Microphthalmia with 52-56kD doublet. Does not crossreact with other b-HLH-ZIP factors by DNA mobility shift assay. Reacts with both melanocytic and non-melanocytic isoforms of Mi. It is especially useful for research on mouse Mi protein. Species Crossreactivity: equine, canine, mouse, rat.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein G affinity chromatography.
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Form Appearance
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M3891X.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage, For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.
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Tested applications
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
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French translation
anticorps