Mouse Anti-Mouse TrpM7 Monoclonal IgG1, Clone S74-25
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Catalog numberSMC-316D-A390
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PricePlease ask
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Size100 µg
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CloneS74-25
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ImmunogenFusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7
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Antibody s full descriptionMouse Anti-Mouse TRPM7 Monoclonal IgG1 Antibody, Clone: S74-25: ATTO 390
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameCHAK antibody, CHAK1 antibody, Channel kinase 1 antibody, Channel-kinase 1 antibody, Long transient receptor potential channel 7 antibody, LTrpC-7 antibody, LTRPC7 antibody, Transient receptor potential cation channel subfamily M member 7 antibody, TRP PLIK antibody, TRPM7 antibody, TRPM7_HUMAN antibody
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Raised inMouse
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Antibody s targetTRPM7
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Primary research fieldsNeuroscience, Ion Channels, Transient Receptor Potential Channels, Neuroscience, Neurodegeneration, Parkinson's Disease, ALS Disease
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~220kDa. No cross-reactivity against TrpM6.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-316 was sufficient for detection of TrpM7 in 10 µg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextTRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants (1, 2).
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Bibliography1. Brauchi S., Krapivinsky G., Krapivinsky L., Clapham D.E. (2008) Proc Natl Acad Sci USA. 105(24): 8304-8308. 2. Numata T, Okada Y. (2008) J Biol Chem. 283(22): 15097-15103.
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Released date6-Jul-2009
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001157797.1
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Gene number58800
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Protein numberQ923J1
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25 (SMC-316). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (SMC-316) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Nuclear staining of both neurons and glia. Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25 (SMC-316). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (SMC-316) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25 (SMC-316). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (SMC-316) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-TrpM7 Antibody [S74-25] used in Immunohistochemistry (IHC) on Mouse Brain Slice (SMC-316) Mouse Anti-TrpM7 Antibody [S74-25] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-316) Mouse Anti-TrpM7 Antibody [S74-25] used in Western Blot (WB) on Human Cell lysates (SMC-316)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD116-25, ZNF211, SNORD114-25, SNORD115-25, ERVW-25, IGKV3D-25, IGLV3-25, RNVU1-25, IGKV3-25, IGHVIII-25-1
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Short nameMouse Anti-Mouse TrpM7 Monoclonal IgG1, Clone S74-25
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 390
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse transient receptor potential cation channel, subfamily M, member 7 monoclonal IgG1, clonality S74-25
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Alternative techniquemurine, antibodies
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Alternative to gene targettransient receptor potential cation channel, subfamily M, member 7, TRPM7 and IDBG-11807 and ENSG00000092439 and 54822, transferase activity, Plasma membranes, Trpm7 and IDBG-204376 and ENSMUSG00000027365 and 58800, TRPM7 and IDBG-646663 and ENSBTAG00000031165 and 514642
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Clone nameS74-25
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-25
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 211
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-29
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Pubmed identfication
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-25
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-25
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 25
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Synonyms gene name
- endogenous retrovirus group W, member 25
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3D-25 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3D-25
- immunoglobulin kappa variable 3D-25 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-25
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 25
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Locus
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Discovery year2019-07-26
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3-25 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3-25
- immunoglobulin kappa variable 3-25 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-25-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-25-1
- immunoglobulin heavy variable (III)-25-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
