Sucrose Colorimetric/Fluorometric Assay Kit
-
Catalog number
K626-100
-
Price
Please ask
-
Size
100 assays
-
-
Description
No interference from Lactose & Maltose
-
Summary
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Applications- The method can detect 0.0002-10 mM sucrose concentrations.
-
Sample Type
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, monitoring level during fermentation and feeding in protein expression processes
-
Features Benefits
• Simple procedure; takes ~1 hour • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Sucrose
-
Storage Conditions
-20°C
-
Shipping Conditions
gel pack
-
Shelf life
12 months
-
Background
Sucrose (C₁₂H₂₂O₁₁; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose. BioVision’s Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.
-
-
Test
Biovision supplies other types of Assays as 1.
-
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
-
Gene target
-
Short name
Sucrose Colorimetric/Fluorometric Assay Kit
-
Technique
colorimetric, Assay, assays, colorimetry
-
Host
Assay
-
Alternative name
Sucrose colorimetry/fluorescence test reagent
-
Alternative technique
assays, arrays, kits
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-
Product images
Similar products